Mouse Anti-PSMD14 Recombinant Antibody (4A10-E8) (CBMAB-0740CQ)

Name: Mouse Anti-PSMD14 Recombinant Antibody (4A10-E8)
SKU: CBMAB-0740CQ
Rating: 5 (1 reviews)

See all PSMD14 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4A10-E8

Application

WB, ELISA, IF

Immunogen

PSMD14 (aa 1-96) full length recombinant protein with GST tag

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

proteasome (prosome, macropain) 26S subunit, non-ATPase, 14

Introduction

This gene encodes a component of the 26S proteasome. a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves Lys-63-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving Lys-63-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.

Entrez Gene ID

10213

UniProt ID

O00487

Alternative Names

PAD1; POH1; RPN11

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.

Biological Process

Double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIMP:UniProtKB
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIMP:UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Protein deubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Protein K63-linked deubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of proteasomal protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Response to ethanolIEA:Ensembl
Ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

cytosol
cytosolic proteasome complex
extracellular region
ficolin-1-rich granule lumen
nucleoplasm
nucleus
proteasome accessory complex
proteasome complex
proteasome regulatory particle, lid subcomplex
secretory granule lumen

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PSMD14 Recombinant Antibody (4A10-E8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PSMD14 Recombinant Antibody (MAD1D6H4) (CAT#: CBMAB-P3223-YC)

Mouse Anti-PSMD14 Recombinant Antibody (9B10) (CAT#: CBMAB-P3220-YC)

Mouse Anti-PSMD14 Recombinant Antibody (CBYC-P714) (CAT#: CBMAB-P3224-YC)

Rabbit Anti-PSMD14 Recombinant Antibody (D18C7) (CAT#: CBMAB-P3221-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry