Rabbit Anti-PTGER2 Recombinant Antibody (
8D8) (V2LY-0725-LY1281)

Name: Rabbit Anti-PTGER2 Recombinant Antibody (8D8)
SKU: V2LY-0725-LY1281
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PTGER2 Recombinant Antibody (8D8) (V2LY-0725-LY1281) in IHC

IHC image of V2LY-0725-LY1281 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PTGER2 Recombinant Antibody (8D8) (V2LY-0725-LY1281) in IHC

IHC image of V2LY-0725-LY1281 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

8D8

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from human PTGER2.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Prostaglandin E Receptor 2

Entrez Gene ID

5732

UniProt ID

P43116

Function

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathwayISS:BHF-UCL
Cellular response to prostaglandin E stimulusISS:BHF-UCL
G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Inflammatory responseManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cytosolic calcium ion concentrationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of cell population proliferationIEA:Ensembl
Response to lipopolysaccharideIEA:Ensembl
Response to nematodeIEA:Ensembl
Response to progesteroneIEA:Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 1-23
Helical: 24-47
Cytoplasmic: 48-65
Helical: 66-91
Extracellular: 92-111
Helical: 112-132
Cytoplasmic: 133-151
Helical: 152-176
Extracellular: 177-198
Helical: 199-223
Cytoplasmic: 224-262
Helical: 263-286
Extracellular: 287-299
Helical: 300-323
Cytoplasmic: 324-358

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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