Rabbit Anti-PTX3 Recombinant Antibody (
10D9) (V2LY-0725-LY1571)

Name: Rabbit Anti-PTX3 Recombinant Antibody (10D9)
SKU: V2LY-0725-LY1571
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PTX3 Recombinant Antibody (10D9) (V2LY-0725-LY1571) in FC

Overlay Peak curve showing A549 cells stained with V2LY-0725-LY1571 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

10D9

Application

ELISA, FC

Immunogen

A synthesized peptide derived from human PTX3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

pentraxin-related gene, rapidly induced by IL-1 beta

Entrez Gene ID

5806

UniProt ID

P26022

Function

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

Biological Process

Extracellular matrix organizationIEA:Ensembl
Inflammatory responseManual Assertion Based On ExperimentTAS:ProtInc
Innate immune responseManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation by host of viral exo-alpha-sialidase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation by host of viral glycoprotein metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation by host of viral processManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of exo-alpha-sialidase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of glycoprotein metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of viral entry into host cellManual Assertion Based On ExperimentIDA:BHF-UCL
OpsonizationIEA:Ensembl
Ovarian cumulus expansionIEA:Ensembl
Positive regulation of nitric oxide biosynthetic processIEA:Ensembl
Positive regulation of phagocytosisIEA:Ensembl
Response to yeastIEA:Ensembl

Cellular Location

Secreted

PTM

Glycosylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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