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Mouse Anti-PXN Recombinant Antibody (5H11) (CBMAB-P3556-YC)

Provided herein is a Mouse monoclonal antibody against Human Paxillin. The antibody can be used for immunoassay techniques, such as IF, IHC-P, IP, WB.
See all PXN antibodies
Published Data
Fig.1 Nicotinic acid (NA) post-transcriptionally downregulates paxillin.
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Fig.2 Paxillin is expressed in specimens of paraffin-embedded glioblastoma multiforme (GBM).
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Summary

Host Animal
Mouse
Specificity
Human
Clone
5H11
Antibody Isotype
IgG1, κ
Application
IF, IHC-P, IP, WB

Basic Information

Immunogen
Purified recombinant full length human paxillin protein
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Paxillin
Introduction
PXN is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties.
Entrez Gene ID
5829
UniProt ID
P49023
Alternative Names
AW123232; Pax; AW108311
Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Biological Process
Cell adhesion1 PublicationNAS:ProtInc
Cell-matrix adhesionIEA:InterPro
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIEP:BHF-UCL
Endothelial cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Growth hormone receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Signal complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIBA:GO_Central
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm, cytoskeleton
Cell junction, focal adhesion
Cytoplasm, cell cortex
Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389).
PTM
Phosphorylated by MAPK1/ERK2 (By similarity).
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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