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Rabbit Anti-PXN Recombinant Antibody (D9G12) (CBMAB-CP1880-LY)

The product is antibody recognizes Paxillin. The antibody D9G12 immunoassay techniques such as: WB,IP.
See all PXN antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
D9G12
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys193 of human paxillin protein.
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Paxillin
Entrez Gene ID
5829
UniProt ID
P49023
Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Biological Process
Cell adhesion1 PublicationNAS:ProtInc
Cell-matrix adhesionIEA:InterPro
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIEP:BHF-UCL
Endothelial cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Growth hormone receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Signal complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIBA:GO_Central
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm, cytoskeleton
Cell junction, focal adhesion
Cytoplasm, cell cortex
Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389).
PTM
Phosphorylated by MAPK1/ERK2 (By similarity).
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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