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Mouse Anti-Rab25 Recombinant Antibody (CBT3670) (V2LY-0625-LY2350)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBT3670
Antibody Isotype
IgG1
Application
WB, IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of Rab25 expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
RAB25, Member RAS Oncogene Family
Entrez Gene ID
57111
UniProt ID
P57735
Function
Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226).
Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity).
May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).
Biological Process
Biological Process epithelial cell morphogenesisISS:UniProtKB
Biological Process exocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of epithelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein transportIEA:UniProtKB-KW
Biological Process pseudopodium organizationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of vesicle-mediated transportManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process vesicle-mediated transportManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell membrane
Cell projection, pseudopodium membrane
Cytoplasmic vesicle
Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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