Mouse Anti-RHOT1 Recombinant Antibody (1A12) (CBMAB-1769-CN)

Name: Mouse Anti-RHOT1 Recombinant Antibody (1A12)
SKU: CBMAB-1769-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1A12

Application

WB, ELISA, IF

Immunogen

RHOT1 (NP_060777, aa. 483-581) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, Κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ras homolog gene family, member T1

Introduction

Mitochondrial Rho GTPase 1 (MIRO1) is an enzyme that in humans is encoded by the RHOT1 gene on chromosome 17. As a Miro protein isoform, the protein facilitates mitochondrial transport by attaching the mitochondria to the motor/adaptor complex. It probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Through its key role in mitochondrial transport, RHOT1 is involved in mitochondrial homeostasis and apoptosis, as well as Parkinson's disease (PD) and cancer.

Entrez Gene ID

55288

UniProt ID

Q8IXI2

Alternative Names

ARHT1; MIRO1; MIRO-1

Function

Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657).
Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657).
Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).

Biological Process

Biological Process cellular homeostasisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitochondrial outer membrane permeabilizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitochondrion organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mitochondrion transport along microtubuleManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of mitochondrion organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process small GTPase mediated signal transductionIEA:InterPro

Cellular Location

Mitochondrion outer membrane
Colocalizes with MGARP and RHOT2 at the mitochondria.

Topology

Cytoplasmic: 1-592
Helical: 593-615
Mitochondrial intermembrane: 616-618

PTM

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy (PubMed:22396657, PubMed:24896179).
Deubiquitinated by USP30 (PubMed:24896179).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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