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Mouse Anti-RHOT1 Recombinant Antibody (1A12) (CBMAB-1769-CN)

This product is a mouse antibody that recognizes RHOT1 of human. The antibody 1A12 can be used for immunoassay techniques such as: WB, ELISA, IF.
See all RHOT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A12
Antibody Isotype
IgG1, Κ
Application
WB, ELISA, IF

Basic Information

Immunogen
RHOT1 (NP_060777, aa. 483-581) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, Κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ras homolog gene family, member T1
Introduction
Mitochondrial Rho GTPase 1 (MIRO1) is an enzyme that in humans is encoded by the RHOT1 gene on chromosome 17. As a Miro protein isoform, the protein facilitates mitochondrial transport by attaching the mitochondria to the motor/adaptor complex. It probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Through its key role in mitochondrial transport, RHOT1 is involved in mitochondrial homeostasis and apoptosis, as well as Parkinson's disease (PD) and cancer.
Entrez Gene ID
55288
UniProt ID
Q8IXI2
Alternative Names
ARHT1; MIRO1; MIRO-1
Function
Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657).
Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657).
Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).
Biological Process
Biological Process cellular homeostasisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitochondrial outer membrane permeabilizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitochondrion organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mitochondrion transport along microtubuleManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of mitochondrion organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process small GTPase mediated signal transductionIEA:InterPro
Cellular Location
Mitochondrion outer membrane
Colocalizes with MGARP and RHOT2 at the mitochondria.
Topology
Cytoplasmic: 1-592
Helical: 593-615
Mitochondrial intermembrane: 616-618
PTM
Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy (PubMed:22396657, PubMed:24896179).
Deubiquitinated by USP30 (PubMed:24896179).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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