Sign in or Register   Sign in or Register
  |  

Mouse Anti-SETDB1 Recombinant Antibody (4A3) (CBMAB-A8016-LY)

The product is antibody recognizes SETDB1. The antibody 4A3 immunoassay techniques such as: WB, ELISA.
See all SETDB1 antibodies
Published Data
Fig.1 Hematoxylin and eosin-stained transverse sections of zebrafish melanomas at two weeks post onset.
...View More
Fig.2 SETDB1abrogates BRAF(V600E)-induced senescence.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
4A3
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
SETDB1 (NP_036564, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
SET Domain Bifurcated 1
Introduction
This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described
Entrez Gene ID
9869
UniProt ID
Q15047
Alternative Names
ESET; KG1T; KIAA0067; KMT1E
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed:12869583).
Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed:27732843, PubMed:14536086).
Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed:14536086,). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306).
Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306).
In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity).
Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306).
The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).
Biological Process
Biological Process heterochromatin organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process histone H3-K9 methylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of single stranded viral RNA replication via double stranded DNA intermediateISS:UniProtKB
Biological Process positive regulation of DNA methylation-dependent heterochromatin assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process Ras protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Chromosome
Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
PTM
Degraded by the proteasome, shielded by interaction with ATF7IP.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-SETDB1 Recombinant Antibody (4A3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare