Mouse Anti-SF1 Recombinant Antibody (CBXS-4525) (CBMAB-S1765-CQ)

Name: Mouse Anti-SF1 Recombinant Antibody (CBXS-4525)
SKU: CBMAB-S1765-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBXS-4525

Application

ELISA, WB, IHC-P, IF

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

splicing factor 1

Introduction

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

7536

UniProt ID

Q15637

Alternative Names

Splicing Factor 1; Zinc Finger Protein 162; Mammalian Branch Point-Binding Protein; Zinc Finger Gene In MEN1 Locus; Transcription Factor ZFM1; ZNF162;

Function

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Biological Process

Biological Process Leydig cell differentiationIEA:Ensembl
Biological Process male sex determinationIEA:Ensembl
Biological Process mRNA 3'-splice site recognitionManual Assertion Based On ExperimentTAS:HGNC-UCL
Biological Process mRNA splicing, via spliceosome1 PublicationIC:HGNC-UCL
Biological Process negative regulation of smooth muscle cell proliferationIEA:Ensembl
Biological Process nuclear body organizationIEA:Ensembl
Biological Process regulation of mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of steroid biosynthetic processIEA:Ensembl
Biological Process spliceosomal complex assembly1 PublicationNAS:UniProtKB

Cellular Location

Nucleus

PTM

Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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