Rabbit Anti-SF3B1 Recombinant Antibody (
6C7) (V2LY-0725-LY939)

Name: Rabbit Anti-SF3B1 Recombinant Antibody (6C7)
SKU: V2LY-0725-LY939
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-SF3B1 Recombinant Antibody (6C7) (V2LY-0725-LY939) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, U-87 whole cell lysate, Mouse Brain whole cell lysate
All lanes: SF3B1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 146, 17 kDa
Observed band size: 130 kDa

Rabbit Anti-SF3B1 Recombinant Antibody (6C7) (V2LY-0725-LY939) in IHC

IHC image of V2LY-0725-LY939 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-SF3B1 Recombinant Antibody (6C7) (V2LY-0725-LY939) in IHC

IHC image of V2LY-0725-LY939 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-SF3B1 Recombinant Antibody (6C7) (V2LY-0725-LY939) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY939 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

6C7

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human SF3B1.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Splicing Factor 3b Subunit 1

Entrez Gene ID

23451

UniProt ID

O75533

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643).
SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).
Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).
May also be involved in the assembly of the 'E' complex (PubMed:10882114).
Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

Biological Process

Chromatin remodeling1 PublicationIC:ComplexPortal
mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of histone acetylation1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase I1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase II1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase IIIManual Assertion Based On ExperimentIDA:ComplexPortal
RNA splicing1 PublicationIC:UniProtKB
RNA splicing, via transesterification reactions1 PublicationNAS:UniProtKB
Spliceosomal complex assemblyManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Nucleus speckle
During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

PTM

Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
Citrullinated by PADI4.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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