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Mouse Anti-SF3B1 Recombinant Antibody (CBXS-3815) (CBMAB-S1086-CQ)

This product is a mouse antibody that recognizes SF3B1. The antibody CBXS-3815 can be used for immunoassay techniques such as: WB.
See all SF3B1 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse
Clone
CBXS-3815
Antibody Isotype
IgG2b
Application
WB

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2, 50% glycerol
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Splicing Factor 3b Subunit 1
Introduction
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Entrez Gene ID
81898
UniProt ID
Q8C839
Alternative Names
155kDa; 2810001M05Rik; AA409119; Prp10; SAP155; SF3b155; TA-8; Targ4
Function
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643).
SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).
Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).
May also be involved in the assembly of the 'E' complex (PubMed:10882114).
Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).
Biological Process
Chromatin remodeling1 PublicationIC:ComplexPortal
mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of histone acetylation1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase I1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase II1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase IIIManual Assertion Based On ExperimentIDA:ComplexPortal
RNA splicing1 PublicationIC:UniProtKB
RNA splicing, via transesterification reactions1 PublicationNAS:UniProtKB
Spliceosomal complex assemblyManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Nucleus speckle
During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
PTM
Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
Citrullinated by PADI4.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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