Rabbit Anti-SF3B1 Recombinant Antibody (CBXS-1299) (CBMAB-S4158-CQ)

Name: Rabbit Anti-SF3B1 Recombinant Antibody (CBXS-1299)
SKU: CBMAB-S4158-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

CBXS-1299

Application

WB, IHC-P, IF

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Splicing Factor 3b Subunit 1

Introduction

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Entrez Gene ID

Human	23451
Mouse	81898
Rat	84486

UniProt ID

Human	O75533
Mouse	Q99NB9
Rat	G3V7T6

Alternative Names

Splicing Factor 3b Subunit 1; Splicing Factor 3b, Subunit 1, 155kDa; Spliceosome-Associated Protein 155; SF3b155; SAP155; Pre-MRNA Splicing Factor SF3b, 155 KDa Subunit; Pre-MRNA-Splicing Factor SF3b 155 KDa Subunit; Splicing Factor 3b, Subunit 1, 155kD;

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643).
SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).
Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).
May also be involved in the assembly of the 'E' complex (PubMed:10882114).
Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

Biological Process

Chromatin remodeling1 PublicationIC:ComplexPortal
mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of histone acetylation1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase I1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase II1 PublicationIC:ComplexPortal
Positive regulation of transcription by RNA polymerase IIIManual Assertion Based On ExperimentIDA:ComplexPortal
RNA splicing1 PublicationIC:UniProtKB
RNA splicing, via transesterification reactions1 PublicationNAS:UniProtKB
Spliceosomal complex assemblyManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Nucleus speckle
During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

PTM

Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
Citrullinated by PADI4.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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