Mouse Anti-SLC27A2 Recombinant Antibody (CBT2284) (V2LY-0625-LY2844)

Mouse

Human

CBT2284

IgG1

IHC

Purified recombinant fragment of human SLC27A2 (AA: 346-405) expressed in E. Coli.

Mouse

Human

IgG1

Monoclonal Antibody

Liquid

PBS

Sodium azide

Batch dependent

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating their transport across cell membranes, playing an important role in hepatic fatty acid uptake (PubMed:20530735, PubMed:22022213, PubMed:24269233, PubMed:10198260, PubMed:10749848, PubMed:11980911).
Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake (PubMed:20530735, PubMed:22022213, PubMed:24269233, PubMed:10198260, PubMed:10749848, PubMed:11980911).
Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis (PubMed:20530735).
Can also activate branched-chain fatty acids such as phytanic acid and pristanic acid (PubMed:10198260).
May contribute to the synthesis of sphingosine-1-phosphate (PubMed:24269233).
Does not activate C24 bile acids, cholate and chenodeoxycholate (PubMed:11980911).
In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol (PubMed:11980911).
However, it is not critical for THCA activation and bile synthesis in vivo (PubMed:20530735).
Isoform 1
Exhibits both long-chain fatty acids (LCFA) transport activity and acyl CoA synthetase towards very long-chain fatty acids (PubMed:21768100, PubMed:10198260).
Shows a preference for generating CoA derivatives of n-3 fatty acids, which are preferentially trafficked into phosphatidylinositol (PubMed:21768100).2 Publications
Isoform 2
Exhibits long-chain fatty acids (LCFA) transport activity but lacks acyl CoA synthetase towards very long-chain fatty acids.

Biological Process bile acid biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process fatty acid alpha-oxidationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process fatty acid beta-oxidationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process long-chain fatty acid import into cellManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process long-chain fatty acid metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process methyl-branched fatty acid metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process very long-chain fatty acid catabolic processManual Assertion Based On ExperimentIBA:GO_Central

Endoplasmic reticulum membrane
Peroxisome membrane
Cell membrane
Microsome

Lumenal: 1-4
Helical: 5-27
Cytoplasmic: 28-106
Helical: 107-127
Lumenal: 128-261
Helical: 262-282
Cytoplasmic: 283-620