Rabbit Anti-SLC2A3 Recombinant Antibody (
14B1) (V2LY-0725-LY984)

Name: Rabbit Anti-SLC2A3 Recombinant Antibody (14B1)
SKU: V2LY-0725-LY984
Rating: 5 (1 reviews)

See all SLC2A3 antibodies

Compare Online Inquiry

Tested Data

Rabbit Anti-SLC2A3 Recombinant Antibody (14B1) (V2LY-0725-LY984) in WB

Western Blot Positive WB detected in: 293 whole cell lysate (20µg), JK whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), SY5Y whole cell lysate (20µg) All lanes: SLC2A3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 60s

Rabbit Anti-SLC2A3 Recombinant Antibody (14B1) (V2LY-0725-LY984) in IHC

IHC image of V2LY-0725-LY984 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-SLC2A3 Recombinant Antibody (14B1) (V2LY-0725-LY984) in IHC

IHC image of V2LY-0725-LY984 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-SLC2A3 Recombinant Antibody (14B1) (V2LY-0725-LY984) in IHC

IHC image of V2LY-0725-LY984 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-SLC2A3 Recombinant Antibody (14B1) (V2LY-0725-LY984) in FC

Overlay Peak curve showing MCF7 cells stained with V2LY-0725-LY984 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

14B1

Application

ELISA, WB, IHC, FC

Immunogen

A synthesized peptide derived from human SLC2A3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Solute Carrier Family 2 Member 3

Entrez Gene ID

6515

UniProt ID

P11169

Function

Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916).
Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916).
Does not mediate fructose transport (PubMed:9477959, PubMed:26176916).

Biological Process

Biological Process carbohydrate metabolic process1 PublicationNAS:ProtInc
Biological Process dehydroascorbic acid transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process galactose transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process glucose importManual Assertion Based On ExperimentIBA:GO_Central
Biological Process glucose import across plasma membraneManual Assertion Based On ExperimentIGI:ARUK-UCL
Biological Process glucose transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process L-ascorbic acid metabolic processTAS:Reactome
Biological Process monosaccharide transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process transport across blood-brain barrierManual Assertion Based On ExperimentIGI:ARUK-UCL

Cellular Location

Cell membrane
Perikaryon
Cell projection
Localized to densely spaced patches along neuronal processes.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-SLC2A3 Recombinant Antibody (
14B1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-SLC2A3 Recombinant Antibody (IHC585) (CAT#: CBMAB-MA225-YC)

Mouse Anti-SLC2A3 (Extracellular Domain) Recombinant Antibody (CBXS-0453) (CAT#: CBMAB-S3403-CQ)

Mouse Anti-SLC2A3 Recombinant Antibody (202017) (CAT#: CBMAB-G1181-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry