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Mouse Anti-SLC32A1 Monoclonal Antibody (CL2793) (CBMAB-1459-YC)

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Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
CL2793
Application
IHC-P
Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human SLC32A1 (aa 13-103). Sequence: ATSVSNKSQAKMSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDILKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQVG.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Solute Carrier Family 32 Member 1
Introduction
SLC32A1 is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles.
Entrez Gene ID
Human140679
Mouse22348
Rat83612
UniProt ID
HumanQ9H598
MouseO35633
RatO35458
Alternative Names
VGAT; VIAAT
Function
Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient (By similarity).
May also transport beta-alanine (By similarity).
Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (By similarity).
Biological Process
Biological Process agingIEA:Ensembl
Biological Process amino acid transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process beta-alanine transportISS:UniProtKB
Biological Process gamma-aminobutyric acid importISS:UniProtKB
Biological Process gamma-aminobutyric acid transportISS:UniProtKB
Biological Process glycine transportISS:UniProtKB
Biological Process hippocampus developmentIEA:Ensembl
Biological Process ion transportTAS:Reactome
Biological Process neurotransmitter loading into synaptic vesicleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process neurotransmitter secretionTAS:Reactome
Cellular Location
Cytoplasmic vesicle membrane
Presynapse
Presents in glycine-, GABA- or GABA- and glycine-containing boutons.
Topology
Cytoplasmic: 1-132
Helical: 133-153
Lumenal: 154-204
Helical: 205-225
Cytoplasmic: 226-265
Helical: 266-286
Lumenal: 287-305
Helical: 306-326
Cytoplasmic: 327-341
Helical: 342-362
Lumenal: 363-383
Helical: 384-404
Cytoplasmic: 405-438
Helical: 439-459
Lumenal: 460-461
Helical: 462-482
Cytoplasmic: 483-489
Helical: 490-510
Lumenal: 490-510
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-SLC32A1 Recombinant Antibody (117G4) (CAT#: CBMAB-V0003-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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