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Rabbit Anti-SMC2 Recombinant Antibody (D11F9) (CBMAB-CP2515-LY)

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Datasheet
SDS
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Basic Information

Host Animal
Rabbit
Clone
D11F9
Application
WB, IP
Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMC2 protein.
Specificity
Human, Mouse, Rat, Monkey, Pig, Horse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
structural maintenance of chromosomes 2
Entrez Gene ID
Human10592
Mouse14211
Rat362519
Monkey713359
Pig100517138
Horse100054338
UniProt ID
HumanO95347
MouseQ8CG48
RatD4A5Q2
MonkeyF6VS74
PigF1SRP0
HorseF7BLC9
Function
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Biological Process
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process kinetochore organizationIEA:Ensembl
Biological Process meiotic chromosome condensationIEA:Ensembl
Biological Process meiotic chromosome segregationIEA:Ensembl
Biological Process mitotic chromosome condensationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of chromosome condensationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process positive regulation of chromosome segregationIEA:Ensembl
Biological Process positive regulation of chromosome separationIEA:Ensembl
Cellular Location
Nucleus
Cytoplasm
Chromosome
In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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