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Mouse Anti-SPDEF Recombinant Antibody (4A5) (CBMAB-A8530-LY)

The product is antibody recognizes SPDEF. The antibody 4A5 immunoassay techniques such as: WB, ELISA.
See all SPDEF antibodies
Published Data
Fig.1 Prostate carcinogenesis is increased in SPDEF2/2 mice.
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Fig.2 Overexpression of SPDEF decreased migration of TRAMP C2 adenocarcinoma cells in vitro. Wound healing assay was used to measure cell migration.
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Fig.3 Prostate carcinogenesis is increased in SPDEF2/4 mice.
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Fig.4 In orthotopic mouse model of prostate cancer, re-expression of Foxm1 in SPDEFoverexpressing cancer cells restored tumor sizes that have been decreased after SPDEF expression (left panel).
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4A5
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
SPDEF (NP_036523, 92 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
SAM pointed domain containing ets transcription factor
Introduction
PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM
Entrez Gene ID
25803
UniProt ID
O95238
Alternative Names
PDEF; RP11-375E1__A.3; bA375E1.3
Function
May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
Biological Process
Biological Process cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process epithelial cell fate commitmentIEA:Ensembl
Biological Process glandular epithelial cell developmentIEA:Ensembl
Biological Process intestinal epithelial cell developmentIEA:Ensembl
Biological Process lung goblet cell differentiationIEA:Ensembl
Biological Process negative regulation of cell fate commitmentIEA:Ensembl
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process positive regulation of cell fate commitmentIEA:Ensembl
Biological Process positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process transcription by RNA polymerase IIIEA:Ensembl
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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