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Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739)


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Tested Data
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in WB
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in WB
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in WB
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in WB
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in FC
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in FC
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in ELISA
Mouse Anti-SPI1 Recombinant Antibody (CBT4079) (V2LY-0625-LY2739) in ELISA

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4079
Antibody Isotype
IgG1
Application
FC

Basic Information

Immunogen
Purified recombinant fragment of human SPI1 (AA: 124-271) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Spi-1 proto-oncogene
Entrez Gene ID
6688
Research Area
Immunology
Function
Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726).
Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
May be particularly important for the pro- to pre-B cell transition (PubMed:33951726).
Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726).
In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
Biological Process
Cell differentiation
Defense response to tumor cell
Endothelial to hematopoietic transition
Interleukin-6-mediated signaling pathway
Myeloid leukocyte differentiation
Negative regulation of adipose tissue development
Negative regulation of DNA-templated transcription
Negative regulation of gene expression
Negative regulation of neutrophil degranulation
Negative regulation of NF-kappaB transcription factor activity
Negative regulation of non-canonical NF-kappaB signal transduction
Negative regulation of protein localization to chromatin
Negative regulation of transcription by RNA polymerase II
Oncogene-induced cell senescence
Pericyte cell differentiation
Positive regulation of antifungal innate immune response
Positive regulation of B cell differentiation
Positive regulation of microglial cell mediated cytotoxicity
Positive regulation of miRNA transcription
Positive regulation of p38MAPK cascade
Positive regulation of transcription by RNA polymerase II
Pro-T cell differentiation
Regulation of DNA-binding transcription factor activity
Regulation of DNA-templated transcription
Regulation of erythrocyte differentiation
Regulation of myeloid progenitor cell differentiation
Regulation of transcription by RNA polymerase II
TRAIL-activated apoptotic signaling pathway
Transcription initiation-coupled chromatin remodeling
Cellular Location
Nucleus
Involvement in disease
Agammaglobulinemia 10, autosomal dominant (AGM10):
A form of agammaglobulinemia, a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
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For research use only. Not intended for any clinical use.

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