Human Recombinant SPI1 protein, His Tag (V2LY-0526-LY6423)

Name: Human Recombinant SPI1 protein, His Tag
SKU: V2LY-0526-LY6423
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SPI1 protein, His Tag consist of Amino Acid: 1-270 and predicts a molecular mass of 32.04 kDa.

Molecule Mass

32.04 kDa

Sequence

Amino Acid: 1-270

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥85% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MES, NaCl, Glycerol, Trehalose, Tween20

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Spi-1 proto-oncogene

Research Area

Immunology

Function

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726).
Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
May be particularly important for the pro- to pre-B cell transition (PubMed:33951726).
Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726).
In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

Biological Process

Cell differentiation
Defense response to tumor cell
Endothelial to hematopoietic transition
Interleukin-6-mediated signaling pathway
Myeloid leukocyte differentiation
Negative regulation of adipose tissue development
Negative regulation of DNA-templated transcription
Negative regulation of gene expression
Negative regulation of neutrophil degranulation
Negative regulation of NF-kappaB transcription factor activity
Negative regulation of non-canonical NF-kappaB signal transduction
Negative regulation of protein localization to chromatin
Negative regulation of transcription by RNA polymerase II
Oncogene-induced cell senescence
Pericyte cell differentiation
Positive regulation of antifungal innate immune response
Positive regulation of B cell differentiation
Positive regulation of microglial cell mediated cytotoxicity
Positive regulation of miRNA transcription
Positive regulation of p38MAPK cascade
Positive regulation of transcription by RNA polymerase II
Pro-T cell differentiation
Regulation of DNA-binding transcription factor activity
Regulation of DNA-templated transcription
Regulation of erythrocyte differentiation
Regulation of myeloid progenitor cell differentiation
Regulation of transcription by RNA polymerase II
TRAIL-activated apoptotic signaling pathway
Transcription initiation-coupled chromatin remodeling

Cellular Location

Nucleus

Involvement in disease

Agammaglobulinemia 10, autosomal dominant (AGM10):
A form of agammaglobulinemia, a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant SPI1 protein, His Tag (CAT#: V2LY-0526-LY8819)

SPI1 Matched Antibody Pair (1131) (CAT#: APMAB-1131LY)

Antibody Pairs

SPI1 Matched Antibody Pair (1131) (CAT#: APMAB-1131LY)

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Mouse Anti-Spi1 Recombinant Antibody (5C7) (CAT#: CBMAB-P3690-YC)

Mouse Anti-SPI1 Recombinant Antibody (CBXS-4611) (CAT#: CBMAB-S1851-CQ)

Mouse Recombinant SPI1 protein, His Tag (CAT#: V2LY-0526-LY8819)

Rabbit Anti-Spi1 Recombinant Antibody (CBYC-P843) (CAT#: CBMAB-P3695-YC)

Mouse Anti-SPI1 Recombinant Antibody (1A3) (CAT#: CBMAB-A8536-LY)

Mouse Anti-Spi1 Recombinant Antibody (CBYC-P841) (CAT#: CBMAB-P3691-YC)

Rabbit Anti-Spi1 Recombinant Antibody (9G7) (CAT#: CBMAB-P3693-YC)

SPI1 Matched Antibody Pair (1131) (CAT#: APMAB-1131LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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