Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-SPI1 Recombinant Antibody (CBXS-3805) (CBMAB-S1076-CQ)


See all SPI1 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-3805
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Spi-1 proto-oncogene
Introduction
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
6688
UniProt ID
P17947
Alternative Names
Spi-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 KDa Transforming Protein; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene; 31 KDa-Transforming Protein; Transcription Factor PU.1;
Research Area
Immunology
Function
Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726).
Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
May be particularly important for the pro- to pre-B cell transition (PubMed:33951726).
Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726).
In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
Biological Process
Cell differentiation
Defense response to tumor cell
Endothelial to hematopoietic transition
Interleukin-6-mediated signaling pathway
Myeloid leukocyte differentiation
Negative regulation of adipose tissue development
Negative regulation of DNA-templated transcription
Negative regulation of gene expression
Negative regulation of neutrophil degranulation
Negative regulation of NF-kappaB transcription factor activity
Negative regulation of non-canonical NF-kappaB signal transduction
Negative regulation of protein localization to chromatin
Negative regulation of transcription by RNA polymerase II
Oncogene-induced cell senescence
Pericyte cell differentiation
Positive regulation of antifungal innate immune response
Positive regulation of B cell differentiation
Positive regulation of microglial cell mediated cytotoxicity
Positive regulation of miRNA transcription
Positive regulation of p38MAPK cascade
Positive regulation of transcription by RNA polymerase II
Pro-T cell differentiation
Regulation of DNA-binding transcription factor activity
Regulation of DNA-templated transcription
Regulation of erythrocyte differentiation
Regulation of myeloid progenitor cell differentiation
Regulation of transcription by RNA polymerase II
TRAIL-activated apoptotic signaling pathway
Transcription initiation-coupled chromatin remodeling
Cellular Location
Nucleus
Involvement in disease
Agammaglobulinemia 10, autosomal dominant (AGM10):
A form of agammaglobulinemia, a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-SPI1 Recombinant Antibody (CBXS-3805)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry