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Rabbit Anti-SYMPK Recombinant Antibody (D5Y3T) (CBMAB-CP2657-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey, Dog, Horse
Clone
D5Y3T
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human symplekin protein.
Specificity
Human, Mouse, Rat, Monkey, Dog, Horse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
symplekin
Entrez Gene ID
Human8189
Mouse68188
Rat292683
Monkey722436
Dog476431
Horse100070949
UniProt ID
HumanQ92797
MouseQ80X82
RatF1LSH0
MonkeyI0FJW9
DogA0A8I3RS81
HorseF6W3K7
Function
Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.
Biological Process
Biological Process cell adhesionIEA:UniProtKB-KW
Biological Process mRNA polyadenylationIMP:UniProtKB1 Publication
Biological Process negative regulation of protein bindingIMP:UniProtKB1 Publication
Biological Process positive regulation of protein dephosphorylationIDA:UniProtKB1 Publication
Cellular Location
Cytoplasm, cytoskeleton
Cell junction, tight junction
Cell membrane
Cell junction
Nucleus, nucleoplasm
Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells. Colocalizes with HSF1 in nuclear stress bodies upon heat shock (PubMed:14707147).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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