Mouse Anti-TAP1 Recombinant Antibody (CBYJT-1959) (CBMAB-T1045-YJ)

Name: Mouse Anti-TAP1 Recombinant Antibody (CBYJT-1959)
SKU: CBMAB-T1045-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYJT-1959

Application

ELISA, WB

Immunogen

TAP1 (AAH14081, 241 a.a. ~ 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: GSETRRLSLF LVLVVLSSLG EMAIPFFTGR LTDWILQDGS ADTFTRNLTL MSILTIASAV LEFVGDGIYN NTMGHVHSHL QGEVFGAVLR QETEFFQQNQ TGNIMSRVTE

Specificity

Human

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 241-350

More Infomation

Target

Full Name

transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)

Introduction

TAP1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). TAP1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. TAP1 is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in TAP1 may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease.

Entrez Gene ID

6890

UniProt ID

Q03518

Alternative Names

Transporter 1, ATP Binding Cassette Subfamily B Member; Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP); Peptide Transporter Involved In Antigen Processing 1; ATP-Binding Cassette Sub-Family B Member 2; Really Interesting New Gene 4 Protein; Peptide Transporter PSF1; Peptide Transporter TAP1; Peptide Supply Factor 1; ABCB2; PSF-1; RING4; APT1; PSF1

Function

ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation.

Biological Process

Adaptive immune response
Antigen processing and presentation of endogenous peptide antigen via MHC class I
Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
Antigen processing and presentation of peptide antigen via MHC class I
Cytosol to endoplasmic reticulum transport
Defense response
Peptide transport
Protein transport
Transmembrane transport
Vesicle fusion with endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane
Viral process

Cellular Location

Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.

Involvement in disease

A HLA class I deficiency. Contrary to bare lymphocyte syndromes type 2 and type 3, which are characterized by early-onset severe combined immunodeficiency, class I antigen deficiencies are not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.

Topology

Cytoplasmic: 61-75 aa
Helical: 76-96 aa
Lumenal: 97-113 aa
Helical: 114-136 aa
Cytoplasmic: 137-152 aa
Helical: 153-173 aa
Lumenal: 174-193 aa
Helical: 194-214 aa
Cytoplasmic: 215-246 aa
Helical: 247-267 aa
Lumenal: 268-287 aa
Helical: 288-308 aa
Cytoplasmic: 309-358 aa
Helical: 359-379 aa
Lumenal: 380-388 aa
Helical: 389-409 aa
Cytoplasmic: 410-478 aa
Helical: 479-499 aa
Lumenal: 500-503 aa
Helical: 504-524 aa
Cytoplasmic: 525-808 aa

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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