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Mouse Anti-TAP1 Recombinant Antibody (53H8) (CBMAB-T1048-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TAP1 (Transporter 1, ATP Binding Cassette Subfamily B Member). The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, IP, WB, FuncS.
See all TAP1 antibodies
Published Data
Fig.1 The expression of β5, β5i, TAP1, Bip, Bax, Bcl-2, Caspase-3 in SN4741 cells after different concentrations of 6-OHDA treatment for 24 h.
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Fig.2 The expression and collocation of TAP1 after 6-OHDA for 1 day in the rat.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
53H8
Antibody Isotype
IgG1
Application
ELISA, ICC, IF, IP, WB, FuncS

Basic Information

Immunogen
Unfortunately, this information is considered to be commercially sensitive
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
0.1% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Introduction
TAP1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). TAP1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. TAP1 is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in TAP1 may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease.
Entrez Gene ID
6890
UniProt ID
Q03518
Alternative Names
Transporter 1, ATP Binding Cassette Subfamily B Member; Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP); Peptide Transporter Involved In Antigen Processing 1; ATP-Binding Cassette Sub-Family B Member 2; Really Interesting New Gene 4 Protein; Peptide Transporter PSF1; Peptide Transporter TAP1; Peptide Supply Factor 1; ABCB2; PSF-1; RING4; APT1; PSF1
Function
ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation.
Biological Process
Adaptive immune response
Antigen processing and presentation of endogenous peptide antigen via MHC class I
Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
Antigen processing and presentation of peptide antigen via MHC class I
Cytosol to endoplasmic reticulum transport
Defense response
Peptide transport
Protein transport
Transmembrane transport
Vesicle fusion with endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane
Viral process
Cellular Location
Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
Involvement in disease
A HLA class I deficiency. Contrary to bare lymphocyte syndromes type 2 and type 3, which are characterized by early-onset severe combined immunodeficiency, class I antigen deficiencies are not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
Topology
Cytoplasmic: 61-75 aa
Helical: 76-96 aa
Lumenal: 97-113 aa
Helical: 114-136 aa
Cytoplasmic: 137-152 aa
Helical: 153-173 aa
Lumenal: 174-193 aa
Helical: 194-214 aa
Cytoplasmic: 215-246 aa
Helical: 247-267 aa
Lumenal: 268-287 aa
Helical: 288-308 aa
Cytoplasmic: 309-358 aa
Helical: 359-379 aa
Lumenal: 380-388 aa
Helical: 389-409 aa
Cytoplasmic: 410-478 aa
Helical: 479-499 aa
Lumenal: 500-503 aa
Helical: 504-524 aa
Cytoplasmic: 525-808 aa
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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