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Mouse Anti-TCF3 Recombinant Antibody (5G2) (CBMAB-A8955-LY)

The product is antibody recognizes TCF3. The antibody 5G2 immunoassay techniques such as: WB, ELISA.
See all TCF3 antibodies
Published Data
Fig. 1 Staining of hippocampal neurons transfected with LAPSER1 RNAi or the empty vector construct revealed 3 h after NMDA application a significant increase of TcfE2a and Myc protein levels in both experimental groups as determined by the measurement of staining intensity within the nuclear compartment of hippocampal neurons.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
5G2
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
TCF3 (NP_003191, 545 a.a. ~ 654 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TCF3 Transcription Factor 3
Introduction
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM
Entrez Gene ID
6929
UniProt ID
P15923
Alternative Names
E2A; ITF1; MGC129647; MGC129648; bHLHb21
Function
Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity).
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity).
Together with TCF15, required for the mesenchymal to epithelial transition (By similarity).
Dimers bind DNA on E-box motifs: 5'-CANNTG-3' (By similarity).
Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed:2493990).
Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).
Isoform E47
Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.
Biological Process
Biological Process B cell differentiationNAS:UniProtKB1 Publication
Biological Process B cell lineage commitmentIDA:UniProtKB1 Publication
Biological Process immunoglobulin V(D)J recombinationIDA:MGI1 Publication
Biological Process negative regulation of transcription by RNA polymerase IIIDA:NTNU_SB1 Publication
Biological Process nervous system developmentIEA:UniProtKB-KW
Biological Process positive regulation of B cell proliferationIMP:UniProtKB
Biological Process positive regulation of cell cycleIDA:UniProtKB
Biological Process positive regulation of DNA-binding transcription factor activityIDA:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcriptionIDA:UniProtKB1 Publication
Biological Process positive regulation of neuron differentiationISS:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIIDA:BHF-UCL1 Publication
Biological Process regulation of DNA-templated transcriptionNAS:UniProtKB1 Publication
Biological Process regulation of G1/S transition of mitotic cell cycleIDA:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIIBA:GO_Central1 Publication
Cellular Location
Nucleus
Involvement in disease
Agammaglobulinemia 8A, autosomal dominant (AGM8A):
A form of agammaglobulinemia, a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
PTM
Phosphorylated following NGF stimulation.
Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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