Mouse Anti-TCF3 Recombinant Antibody (CBYJT-2257) (CBMAB-T1366-YJ)

Name: Mouse Anti-TCF3 Recombinant Antibody (CBYJT-2257)
SKU: CBMAB-T1366-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYJT-2257

Application

ELISA, WB

Immunogen

Partial recombinant corresponding to aa545-655 from human TCF3 (NP_003191) with GST tag. MW of the GST tag alone is 26kD

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 545-655

More Infomation

Target

Full Name

TCF3 Transcription Factor 3

Introduction

TCF3 is a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may function in lymphoid malignancies. TCF3 is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1; 19), with PBX1), childhood leukemia (t(19; 19), with TFPT) and acute leukemia (t(12; 19), with ZNF384).

Entrez Gene ID

6929

UniProt ID

P15923

Alternative Names

Transcription Factor 3; Immunoglobulin Transcription Factor 1; Kappa-E2-Binding Factor; Class B Basic Helix-Loop-Helix Protein 21; Transcription Factor E2-Alpha; Transcription Factor ITF-1; VDR Interacting Repressor; BHLHb21; TCF-3; ITF1; E2A; Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47)

Function

Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity).
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity).
Together with TCF15, required for the mesenchymal to epithelial transition (By similarity).
Dimers bind DNA on E-box motifs: 5'-CANNTG-3' (By similarity).
Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed:2493990).
Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).
Isoform E47
Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.

Biological Process

Biological Process B cell differentiationNAS:UniProtKB1 Publication
Biological Process B cell lineage commitmentIDA:UniProtKB1 Publication
Biological Process immunoglobulin V(D)J recombinationIDA:MGI1 Publication
Biological Process negative regulation of transcription by RNA polymerase IIIDA:NTNU_SB1 Publication
Biological Process nervous system developmentIEA:UniProtKB-KW
Biological Process positive regulation of B cell proliferationIMP:UniProtKB
Biological Process positive regulation of cell cycleIDA:UniProtKB
Biological Process positive regulation of DNA-binding transcription factor activityIDA:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcriptionIDA:UniProtKB1 Publication
Biological Process positive regulation of neuron differentiationISS:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIIDA:BHF-UCL1 Publication
Biological Process regulation of DNA-templated transcriptionNAS:UniProtKB1 Publication
Biological Process regulation of G1/S transition of mitotic cell cycleIDA:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIIBA:GO_Central1 Publication

Cellular Location

Nucleus

Involvement in disease

Agammaglobulinemia 8A, autosomal dominant (AGM8A):
A form of agammaglobulinemia, a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.

PTM

Phosphorylated following NGF stimulation.
Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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