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Rat Anti-TCF3 Recombinant Antibody (CBYJT-2256) (CBMAB-T1365-YJ)

Provided herein is a Rat monoclonal antibody, which binds to TCF3 (Transcription Factor 3). The antibody can be used for immunoassay techniques, such as ICC.
See all TCF3 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
CBYJT-2256
Antibody Isotype
IgG2b
Application
ICC

Basic Information

Immunogen
E. coli-derived recombinant human TCF-3/E2A, Gly100-Glu500, Accession # NP_003191
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, Trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 100-500

Target

Full Name
TCF3 Transcription Factor 3
Introduction
TCF3 is a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may function in lymphoid malignancies. TCF3 is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1; 19), with PBX1), childhood leukemia (t(19; 19), with TFPT) and acute leukemia (t(12; 19), with ZNF384).
Entrez Gene ID
6929
UniProt ID
P15923
Alternative Names
Transcription Factor 3; Immunoglobulin Transcription Factor 1; Kappa-E2-Binding Factor; Class B Basic Helix-Loop-Helix Protein 21; Transcription Factor E2-Alpha; Transcription Factor ITF-1; VDR Interacting Repressor; BHLHb21; TCF-3; ITF1; E2A; Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47)
Function
Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity).
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity).
Together with TCF15, required for the mesenchymal to epithelial transition (By similarity).
Dimers bind DNA on E-box motifs: 5'-CANNTG-3' (By similarity).
Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed:2493990).
Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).
Isoform E47
Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.
Biological Process
Biological Process B cell differentiationNAS:UniProtKB1 Publication
Biological Process B cell lineage commitmentIDA:UniProtKB1 Publication
Biological Process immunoglobulin V(D)J recombinationIDA:MGI1 Publication
Biological Process negative regulation of transcription by RNA polymerase IIIDA:NTNU_SB1 Publication
Biological Process nervous system developmentIEA:UniProtKB-KW
Biological Process positive regulation of B cell proliferationIMP:UniProtKB
Biological Process positive regulation of cell cycleIDA:UniProtKB
Biological Process positive regulation of DNA-binding transcription factor activityIDA:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcriptionIDA:UniProtKB1 Publication
Biological Process positive regulation of neuron differentiationISS:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIIDA:BHF-UCL1 Publication
Biological Process regulation of DNA-templated transcriptionNAS:UniProtKB1 Publication
Biological Process regulation of G1/S transition of mitotic cell cycleIDA:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIIBA:GO_Central1 Publication
Cellular Location
Nucleus
Involvement in disease
Agammaglobulinemia 8A, autosomal dominant (AGM8A):
A form of agammaglobulinemia, a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
PTM
Phosphorylated following NGF stimulation.
Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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