Mouse Anti-MED16 Recombinant Antibody (3A3) (CBMAB-A9084-LY)

Name: Mouse Anti-MED16 Recombinant Antibody (3A3)
SKU: CBMAB-A9084-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

3A3

Antibody Isotype

IgG2b, κ

Application

WB, ELISA

Basic Information

Immunogen

THRAP5 (AAH17282, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Mediator Complex Subunit 16

Entrez Gene ID

Human	10025
Mouse	216154
Rat	299607

UniProt ID

Human	Q9Y2X0
Mouse	Q6PGF3
Rat	D3ZHA9

Alternative Names

DRIP92; THRAP5; TRAP95

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Transcription by RNA polymerase II Source: ProtInc

Cellular Location

Nucleus

More Infomation

References

Guo, P., Chong, L., Wu, F., Hsu, C. C., Li, C., Zhu, J. K., & Zhu, Y. (2021). Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis. Journal of Integrative Plant Biology, 63(4), 802-815.

Lee, M., Dominguez-Ferreras, A., Kaliyadasa, E., Huang, W. J., Antony, E., Stevenson, T., ... & Knight, H. (2021). Mediator subunits MED16, MED14, and MED2 are required for activation of ABRE-dependent transcription in Arabidopsis. Frontiers in Plant Science, 12, 649720.

Gao, H., Bai, P., Xiao, L., Shen, M., Yu, Q., Lei, Y., ... & Bu, H. (2020). Mediator complex subunit 16 is down-regulated in papillary thyroid cancer, leading to increased transforming growth factor-β signaling and radioiodine resistance. Journal of Biological Chemistry, 295(31), 10726-10740.

Liu, Z., Chen, G., Gao, F., Xu, R., Li, N., Zhang, Y., & Li, Y. (2019). Transcriptional repression of the APC/C activator genes CCS52A1/A2 by the mediator complex subunit MED16 controls endoreduplication and cell growth in Arabidopsis. The Plant Cell, 31(8), 1899-1912.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

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Mouse Anti-MED16 Recombinant Antibody (3F5)

Mouse Anti-MED16 Recombinant Antibody (CBYJT-1463)

Mouse Anti-MED16 Recombinant Antibody (2B7)

Mouse Anti-MED16 Recombinant Antibody (3B10)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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