Rabbit Anti-TK1 Recombinant Antibody (
6F6) (V2LY-0725-LY1489)

Name: Rabbit Anti-TK1 Recombinant Antibody (6F6)
SKU: V2LY-0725-LY1489
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-TK1 Recombinant Antibody (6F6) (V2LY-0725-LY1489) in WB

Western Blot
Positive WB detected in: 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate
All lanes: Thymidine Kinase 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa

Rabbit Anti-TK1 Recombinant Antibody (6F6) (V2LY-0725-LY1489) in IF

Immunofluorescence staining of Hela with V2LY-0725-LY1489 at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 491-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-TK1 Recombinant Antibody (6F6) (V2LY-0725-LY1489) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY1489 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

6F6

Application

ELISA, WB, IF, FC

Immunogen

A synthesized peptide derived from Human TK1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

thymidine kinase 1

Entrez Gene ID

7083

UniProt ID

P04183

Function

Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed:9575153).
Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed:22385435).
Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed:22385435).

Biological Process

Biological Process DNA biosynthetic processSource:UniProtKB-KW
Biological Process nucleobase-containing compound metabolic processSource:ProtInc1 Publication
Biological Process phosphorylationSource:UniProtKB-KW
Biological Process protein homotetramerizationSource:UniProtKB1 Publication
Biological Process thymidine metabolic processSource:UniProtKB1 Publication

Cellular Location

Cytoplasm

PTM

Phosphorylated on Ser-13 in mitosis. Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level (PubMed:9575153, PubMed:14697231).
Polyubiquitinated. Postmitosis, ubiquitination leads to proteasomal degradation. The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

TK1 Matched Antibody Pair (1209) (CAT#: APMAB-1209LY)

TK1 Matched Antibody Pair (1210) (CAT#: APMAB-1210LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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