Mouse Anti-TK1 Recombinant Antibody (CBYJT-3266) (CBMAB-T2551-YJ)

Name: Mouse Anti-TK1 Recombinant Antibody (CBYJT-3266)
SKU: CBMAB-T2551-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYJT-3266

Application

WB, IHC, IP, ELISA

Immunogen

Recombinant human p53 expressed in E. coli

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

thymidine kinase 1

Introduction

TK1 is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. TK1, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.

Entrez Gene ID

7083

UniProt ID

P04183

Alternative Names

Thymidine Kinase 1; EC 2.7.1.21; Thymidine Kinase 1 Soluble Isoform; Thymidine Kinase 1, Soluble; Thymidine Kinase, Cytosolic; TK2

Function

Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed:9575153).
Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed:22385435).
Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed:22385435).

Biological Process

Biological Process DNA biosynthetic processSource:UniProtKB-KW
Biological Process nucleobase-containing compound metabolic processSource:ProtInc1 Publication
Biological Process phosphorylationSource:UniProtKB-KW
Biological Process protein homotetramerizationSource:UniProtKB1 Publication
Biological Process thymidine metabolic processSource:UniProtKB1 Publication

Cellular Location

Cytoplasm

PTM

Phosphorylated on Ser-13 in mitosis. Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level (PubMed:9575153, PubMed:14697231).
Polyubiquitinated. Postmitosis, ubiquitination leads to proteasomal degradation. The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

TK1 Matched Antibody Pair (1209) (CAT#: APMAB-1209LY)

TK1 Matched Antibody Pair (1210) (CAT#: APMAB-1210LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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