Rabbit Anti-TLR5 Recombinant Antibody (
10D8) (V2LY-0725-LY1218)
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Positive WB detected in: THP-1 whole cell lysate, RAW264.7 whole cell lysate, U-251 whole cell lysate, K562 whole cell lysate
All lanes: TLR5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
Basic Information
| Application | Note |
| WB | 1:500-1:5,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed:29934223).
Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed:20855887, PubMed:11489966).
Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).
Biological Process defense response to bacteriumSource:InterPro
Biological Process inflammatory responseSource:GO_Central1 Publication
Biological Process innate immune responseSource:UniProtKB-KW
Biological Process MyD88-dependent toll-like receptor signaling pathwaySource:InterPro
Biological Process positive regulation of interleukin-8 productionSource:BHF-UCL1 Publication
Biological Process positive regulation of toll-like receptor signaling pathwaySource:BHF-UCL1 Publication
Biological Process toll-like receptor 5 signaling pathwaySource:InterPro
Biological Process toll-like receptor signaling pathwaySource:GO_Central1 Publication
Helical: 640-660
Cytoplasmic: 661-858
10D8)?
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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