Rabbit Anti-TOMM22 Recombinant Antibody (
7C11) (V2LY-0725-LY1153)

Name: Rabbit Anti-TOMM22 Recombinant Antibody (7C11)
SKU: V2LY-0725-LY1153
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-TOMM22 Recombinant Antibody (7C11) (V2LY-0725-LY1153) in WB

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: TOMM22 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa

Rabbit Anti-TOMM22 Recombinant Antibody (7C11) (V2LY-0725-LY1153) in IHC

IHC image of V2LY-0725-LY1153 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

7C11

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human TOMM22.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

translocase of outer mitochondrial membrane 22 homolog (yeast)

Entrez Gene ID

56993

UniProt ID

Q9NS69

Function

Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (PubMed:10982837).
Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

Biological Process

Biological Process positive regulation of apoptotic process Source:Ensembl
Biological Process protein insertion into mitochondrial outer membrane Source:BHF-UCL1 Publication
Biological Process protein targeting to mitochondrion Source:HGNC-UCL1 Publication

Cellular Location

Mitochondrion outer membrane

Topology

Cytoplasmic: 2-83
Helical: 84-103
Mitochondrial intermembrane: 104-142

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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