Mouse Anti-Top2 Recombinant Antibody (CBYJT-4200) (CBMAB-T3657-YJ)

Name: Mouse Anti-Top2 Recombinant Antibody (CBYJT-4200)
SKU: CBMAB-T3657-YJ
Rating: 5 (1 reviews)

See all Top2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-4200

Application

Specificity

Fruit fly

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Topoisomerase 2

Introduction

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614). Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289). Top2 is essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780). During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780). During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348). Top2 may have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780). Top2 may be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663). It regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).

Entrez Gene ID

35225

UniProt ID

P15348

Alternative Names

CG10223; CHRAC; dCHRAC; Dmel\CG10223; dTopoII; suo; top2; TOP2; TopII; topo II; Topo II; TOPO II; topoII; TopoII; TOPOII

Function

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614).
Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289).
Essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780).
During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780).
During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348).
May have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780).
May be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663).
Regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).

Biological Process

Biological Process chromosome condensation Source:FlyBase1 Publication
Biological Process DNA topological change Source:FlyBase2 Publications
Biological Process heterochromatin assembly Source:FlyBase1 Publication
Biological Process male meiosis chromosome segregation Source:FlyBase1 Publication
Biological Process meiotic cell cycle Source:FlyBase1 Publication
Biological Process metaphase plate congression Source:FlyBase1 Publication
Biological Process mitotic cell cycle Source:FlyBase1 Publication
Biological Process mitotic chromosome condensation Source:FlyBase1 Publication
Biological Process mitotic sister chromatid segregation Source:FlyBase1 Publication
Biological Process resolution of meiotic recombination intermediates Source:GO_Central1 Publication
Biological Process sister chromatid segregation Source:FlyBase2 Publications

Cellular Location

Nucleus
Chromosome
Cytoplasm
Nuclear at interphase, becoming diffusely dispersed in the cytoplasm at later cell cycle stages (PubMed:10885744, PubMed:10751154).
However in early developing embryos, some may remain associated with condensed chromosomes at metaphase (PubMed:10885744).

PTM

Phosphorylated (PubMed:1328202, PubMed:8383533, PubMed:10751154).
Phosphorylation by casein kinase II enhances ATPase activity (PubMed:1328202).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-Top2 Recombinant Antibody (CBYJT-4200)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-Top2 Recombinant Antibody (CBYJT-4201) (CAT#: CBMAB-T3658-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry