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Mouse Anti-Top2 Recombinant Antibody (CBYJT-4201) (CBMAB-T3658-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Top2 (Topoisomerase 2). The antibody can be used for immunoassay techniques, such as WB, IP.
See all Top2 antibodies

Summary

Host Animal
Mouse
Specificity
Fruit fly
Clone
CBYJT-4201
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Specificity
Fruit fly
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Topoisomerase 2
Introduction
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614). Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289). Top2 is essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780). During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780). During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348). Top2 may have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780). Top2 may be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663). It regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).
Entrez Gene ID
UniProt ID
Alternative Names
CG10223; CHRAC; dCHRAC; Dmel\CG10223; dTopoII; suo; top2; TOP2; TopII; topo II; Topo II; TOPO II; topoII; TopoII; TOPOII
Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614).
Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289).
Essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780).
During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780).
During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348).
May have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780).
May be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663).
Regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).
Biological Process
Biological Process chromosome condensation Source:FlyBase1 Publication
Biological Process DNA topological change Source:FlyBase2 Publications
Biological Process heterochromatin assembly Source:FlyBase1 Publication
Biological Process male meiosis chromosome segregation Source:FlyBase1 Publication
Biological Process meiotic cell cycle Source:FlyBase1 Publication
Biological Process metaphase plate congression Source:FlyBase1 Publication
Biological Process mitotic cell cycle Source:FlyBase1 Publication
Biological Process mitotic chromosome condensation Source:FlyBase1 Publication
Biological Process mitotic sister chromatid segregation Source:FlyBase1 Publication
Biological Process resolution of meiotic recombination intermediates Source:GO_Central1 Publication
Biological Process sister chromatid segregation Source:FlyBase2 Publications
Cellular Location
Nucleus
Chromosome
Cytoplasm
Nuclear at interphase, becoming diffusely dispersed in the cytoplasm at later cell cycle stages (PubMed:10885744, PubMed:10751154).
However in early developing embryos, some may remain associated with condensed chromosomes at metaphase (PubMed:10885744).
PTM
Phosphorylated (PubMed:1328202, PubMed:8383533, PubMed:10751154).
Phosphorylation by casein kinase II enhances ATPase activity (PubMed:1328202).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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