Mouse Anti-Top2 Recombinant Antibody (CBYJT-4201) (CBMAB-T3658-YJ)

Name: Mouse Anti-Top2 Recombinant Antibody (CBYJT-4201)
SKU: CBMAB-T3658-YJ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBYJT-4201

Application

WB, IP

Specificity

Fruit fly

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Topoisomerase 2

Introduction

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614). Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289). Top2 is essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780). During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780). During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348). Top2 may have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780). Top2 may be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663). It regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).

Entrez Gene ID

35225

UniProt ID

P15348

Alternative Names

CG10223; CHRAC; dCHRAC; Dmel\CG10223; dTopoII; suo; top2; TOP2; TopII; topo II; Topo II; TOPO II; topoII; TopoII; TOPOII

Function

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:8978614).
Topoisomerase II makes double-strand breaks (PubMed:6308011, PubMed:2547764, PubMed:1328202, PubMed:8383533, PubMed:10786800, PubMed:9545289).
Essential during mitosis and meiosis for proper segregation of daughter chromosomes (PubMed:10751154, PubMed:14600258, PubMed:18752348, PubMed:25340780).
During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I (PubMed:25340780).
During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles (PubMed:18752348).
May have a role in chromatin condensation and chromosome structure (PubMed:14600258, PubMed:18752348, PubMed:25340780).
May be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes (PubMed:23989663).
Regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation (PubMed:21304601).

Biological Process

Biological Process chromosome condensation Source:FlyBase1 Publication
Biological Process DNA topological change Source:FlyBase2 Publications
Biological Process heterochromatin assembly Source:FlyBase1 Publication
Biological Process male meiosis chromosome segregation Source:FlyBase1 Publication
Biological Process meiotic cell cycle Source:FlyBase1 Publication
Biological Process metaphase plate congression Source:FlyBase1 Publication
Biological Process mitotic cell cycle Source:FlyBase1 Publication
Biological Process mitotic chromosome condensation Source:FlyBase1 Publication
Biological Process mitotic sister chromatid segregation Source:FlyBase1 Publication
Biological Process resolution of meiotic recombination intermediates Source:GO_Central1 Publication
Biological Process sister chromatid segregation Source:FlyBase2 Publications

Cellular Location

Nucleus
Chromosome
Cytoplasm
Nuclear at interphase, becoming diffusely dispersed in the cytoplasm at later cell cycle stages (PubMed:10885744, PubMed:10751154).
However in early developing embryos, some may remain associated with condensed chromosomes at metaphase (PubMed:10885744).

PTM

Phosphorylated (PubMed:1328202, PubMed:8383533, PubMed:10751154).
Phosphorylation by casein kinase II enhances ATPase activity (PubMed:1328202).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-Top2 Recombinant Antibody (CBYJT-4200) (CAT#: CBMAB-T3657-YJ)

Isotype control

For research use only. Not intended for any clinical use.

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