Rabbit Anti-TOP2A Recombinant Antibody (
5B10) (V2LY-0725-LY1336)

Name: Rabbit Anti-TOP2A Recombinant Antibody (5B10)
SKU: V2LY-0725-LY1336
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-TOP2A Recombinant Antibody (5B10) (V2LY-0725-LY1336) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: TOP2A antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 175, 178, 179, 183 kDa
Observed band size: 175 kDa

Rabbit Anti-TOP2A Recombinant Antibody (5B10) (V2LY-0725-LY1336) in IHC

IHC image of V2LY-0725-LY1336 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

5B10

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DNA Topoisomerase II Alpha

Entrez Gene ID

7153

Function

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

Biological Process

Apoptotic chromosome condensationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
DNA ligationManual Assertion Based On ExperimentIDA:UniProtKB
DNA topological changeManual Assertion Based On ExperimentIDA:UniProtKB
Embryonic cleavageIEA:Ensembl
Female meiotic nuclear divisionIEA:Ensembl
Hematopoietic progenitor cell differentiationIEA:Ensembl
Negative regulation of DNA duplex unwindingManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of single stranded viral RNA replication via double stranded DNA intermediateManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Regulation of circadian rhythmISS:UniProtKB
Resolution of meiotic recombination intermediatesManual Assertion Based On ExperimentIBA:GO_Central
Rhythmic processIEA:UniProtKB-KW
Sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Nucleus, nucleoplasm
Nucleus
Nucleus, nucleolus

PTM

Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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