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Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386)


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Tested Data
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in ELISA
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in ELISA
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in WB
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in WB
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in WB
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in WB
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in WB
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in WB
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in IF
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in IF
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in IF
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in IF
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in FC
Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (V2LY-0625-LY1386) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4204
Antibody Isotype
IgG2b
Application
WB, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ICC1:100-1:500
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
7153
Function
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Biological Process
Apoptotic chromosome condensationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
DNA ligationManual Assertion Based On ExperimentIDA:UniProtKB
DNA topological changeManual Assertion Based On ExperimentIDA:UniProtKB
Embryonic cleavageIEA:Ensembl
Female meiotic nuclear divisionIEA:Ensembl
Hematopoietic progenitor cell differentiationIEA:Ensembl
Negative regulation of DNA duplex unwindingManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of single stranded viral RNA replication via double stranded DNA intermediateManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Regulation of circadian rhythmISS:UniProtKB
Resolution of meiotic recombination intermediatesManual Assertion Based On ExperimentIBA:GO_Central
Rhythmic processIEA:UniProtKB-KW
Sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Nucleus, nucleoplasm
Nucleus
Nucleus, nucleolus
PTM
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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