Human TOP2A ELISA Kit (V2LY-0626-LY5588)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0098 ng/mL
Detection Range
0.02-3.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
174.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
DNA Topoisomerase II Alpha
Function
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Biological Process
Apoptotic chromosome condensationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
DNA ligationManual Assertion Based On ExperimentIDA:UniProtKB
DNA topological changeManual Assertion Based On ExperimentIDA:UniProtKB
Embryonic cleavageIEA:Ensembl
Female meiotic nuclear divisionIEA:Ensembl
Hematopoietic progenitor cell differentiationIEA:Ensembl
Negative regulation of DNA duplex unwindingManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of single stranded viral RNA replication via double stranded DNA intermediateManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Regulation of circadian rhythmISS:UniProtKB
Resolution of meiotic recombination intermediatesManual Assertion Based On ExperimentIBA:GO_Central
Rhythmic processIEA:UniProtKB-KW
Sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Nucleus, nucleoplasm
Nucleus
Nucleus, nucleolus
PTM
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Top2a ELISA Kit (CAT#: V2LY-0626-LY1564)

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Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4204) (CAT#: CBMAB-T3661-YJ)

Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4205) (CAT#: CBMAB-T3662-YJ)

Mouse Anti-TOP2A Recombinant Antibody (CBT4204) (CAT#: V2LY-0625-LY1386)

Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4210) (CAT#: CBMAB-T3667-YJ)

Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4207) (CAT#: CBMAB-T3664-YJ)

Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4206) (CAT#: CBMAB-T3663-YJ)

Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4211) (CAT#: CBMAB-T3668-YJ)

Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4202) (CAT#: CBMAB-T3659-YJ)

Isotype control

For research use only. Not intended for any clinical use.

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