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Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4211) (CBMAB-T3668-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TOP2A (DNA Topoisomerase II Alpha). The antibody can be used for immunoassay techniques, such as FC, ICC, IHC, IP, WB.
See all TOP2A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4211
Antibody Isotype
IgG2a
Application
FC, ICC, IHC, IP, WB

Basic Information

Immunogen
Nuclear protein preparation from the human cell line U937.50
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.6
Preservative
0.09% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Topoisomerase II Alpha
Introduction
TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. TOP2A catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. TOP2A plays a role as the target for several anticancer agents and a variety of mutations in TOP2A have been associated with the development of drug resistance.
Entrez Gene ID
7153
UniProt ID
P11388
Alternative Names
DNA Topoisomerase II Alpha; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; EC 5.99.1.3; TOP2; DNA Topoisomerase (ATP-Hydrolyzing)
Function
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Biological Process
Apoptotic chromosome condensationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
DNA ligationManual Assertion Based On ExperimentIDA:UniProtKB
DNA topological changeManual Assertion Based On ExperimentIDA:UniProtKB
Embryonic cleavageIEA:Ensembl
Female meiotic nuclear divisionIEA:Ensembl
Hematopoietic progenitor cell differentiationIEA:Ensembl
Negative regulation of DNA duplex unwindingManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of single stranded viral RNA replication via double stranded DNA intermediateManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Regulation of circadian rhythmISS:UniProtKB
Resolution of meiotic recombination intermediatesManual Assertion Based On ExperimentIBA:GO_Central
Rhythmic processIEA:UniProtKB-KW
Sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Nucleus, nucleoplasm
Nucleus
Nucleus, nucleolus
PTM
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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