Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4207) (CBMAB-T3664-YJ)

Name: Mouse Anti-TOP2A Recombinant Antibody (CBYJT-4207)
SKU: CBMAB-T3664-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYJT-4207

Application

ELISA, IF, IHC-P, WB

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DNA Topoisomerase II Alpha

Introduction

TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. TOP2A catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. TOP2A plays a role as the target for several anticancer agents and a variety of mutations in TOP2A have been associated with the development of drug resistance.

Entrez Gene ID

7153

UniProt ID

P11388

Alternative Names

DNA Topoisomerase II Alpha; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; EC 5.99.1.3; TOP2; DNA Topoisomerase (ATP-Hydrolyzing)

Function

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

Biological Process

Apoptotic chromosome condensationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
DNA ligationManual Assertion Based On ExperimentIDA:UniProtKB
DNA topological changeManual Assertion Based On ExperimentIDA:UniProtKB
Embryonic cleavageIEA:Ensembl
Female meiotic nuclear divisionIEA:Ensembl
Hematopoietic progenitor cell differentiationIEA:Ensembl
Negative regulation of DNA duplex unwindingManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of single stranded viral RNA replication via double stranded DNA intermediateManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Regulation of circadian rhythmISS:UniProtKB
Resolution of meiotic recombination intermediatesManual Assertion Based On ExperimentIBA:GO_Central
Rhythmic processIEA:UniProtKB-KW
Sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Nucleus, nucleoplasm
Nucleus
Nucleus, nucleolus

PTM

Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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