Rabbit Anti-TPSB2 Recombinant Antibody (
9H6) (V2LY-0725-LY1529)

Name: Rabbit Anti-TPSB2 Recombinant Antibody (9H6)
SKU: V2LY-0725-LY1529
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-TPSB2 Recombinant Antibody (9H6) (V2LY-0725-LY1529) in WB

Western Blot
Positive WB detected in: Mouse Kidney tissue lysate,
All lanes: TPSB2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa

Rabbit Anti-TPSB2 Recombinant Antibody (9H6) (V2LY-0725-LY1529) in FC

Overlay Peak curve showing K562 cells stained with V2LY-0725-LY1529 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

9H6

Application

ELISA, WB, FC

Immunogen

A synthesized peptide derived from Human TPSB2.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Tryptase Beta 2

Entrez Gene ID

64499

UniProt ID

P20231

Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.

Biological Process

Biological Process proteolysis Source:ComplexPortal1 Publication

Cellular Location

Secreted
Released from the secretory granules upon mast cell activation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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