Rabbit Anti-TPT1 Recombinant Antibody (
10A9) (V2LY-0725-LY1051)

Name: Rabbit Anti-TPT1 Recombinant Antibody (10A9)
SKU: V2LY-0725-LY1051
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-TPT1 Recombinant Antibody (10A9) (V2LY-0725-LY1051) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue
All lanes: TPT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa

Rabbit Anti-TPT1 Recombinant Antibody (10A9) (V2LY-0725-LY1051) in IHC

IHC image of V2LY-0725-LY1051 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-TPT1 Recombinant Antibody (10A9) (V2LY-0725-LY1051) in IF

Immunofluorescence staining of SY5Y Cells with V2LY-0725-LY1051 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-TPT1 Recombinant Antibody (10A9) (V2LY-0725-LY1051) in IP

Immunoprecipitating TCTP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY1051 in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY1051(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

10A9

Application

ELISA, WB, IHC, IF, IP

Immunogen

A synthesized peptide derived from human TCTP.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

TPT1 Gene(Protein Coding) Tumor Protein, Translationally-Controlled 1

Entrez Gene ID

7178

UniProt ID

P13693

Function

Involved in calcium binding and microtubule stabilization.

Biological Process

Biological Process calcium ion transport Source:UniProtKB1 Publication
Biological Process cellular calcium ion homeostasis Source:UniProtKB1 Publication
Biological Process negative regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process negative regulation of ectoderm development Source:Ensembl
Biological Process negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source:MGI1 Publication
Biological Process regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process response to virus Source:UniProtKB1 Publication
Biological Process stem cell population maintenance Source:Ensembl

Cellular Location

Cytoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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