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Mouse Anti-TRIB3 Recombinant Antibody (CBYJT-4660) (CBMAB-T4194-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TRIB3 (Tribbles Pseudokinase 3). The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all TRIB3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4660
Antibody Isotype
IgG1, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
Recombinant protein corresponding to aa56-145 from human TRIB3 with GST tag. MW of the GST tag alone is 26kD
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 56-145

Target

Full Name
TRIB3 Gene(Protein Coding) Tribbles Pseudokinase 3
Introduction
TRIB3 is a putative protein kinase that is induced by the transcription factor NF-kappaB. TRIB3 is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, it can negatively regulate the cell survival serine-threonine kinase AKT1.
Entrez Gene ID
57761
UniProt ID
Q96RU7
Alternative Names
Tribbles Pseudokinase 3; P65-Interacting Inhibitor Of NF-Kappa-B; C20orf97; SKIP3; TRB-3; TRB3; NIPK; SINK
Function
Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:15781252, PubMed:15775988).
Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed:15781252, PubMed:15775988).
May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed:15781252, PubMed:15775988).
Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity).
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity).
May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity).
Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed:12736262).
Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed:15299019).
Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230).
Biological Process
Biological Process cellular response to insulin stimulus Source:BHF-UCLBy Similarity
Biological Process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source:UniProtKB1 Publication
Biological Process negative regulation of DNA-templated transcription Source:UniProtKB1 Publication
Biological Process negative regulation of fat cell differentiation Source:BHF-UCLBy Similarity
Biological Process negative regulation of fatty acid biosynthetic process Source:BHF-UCLBy Similarity
Biological Process negative regulation of protein kinase activity Source:BHF-UCL1 Publication
Biological Process negative regulation of transcription by RNA polymerase II Source:ParkinsonsUK-UCL1 Publication
Biological Process positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source:GO_Central1 Publication
Biological Process positive regulation of protein binding Source:BHF-UCLBy Similarity
Biological Process positive regulation of ubiquitin-protein transferase activity Source:BHF-UCLBy Similarity
Biological Process protein phosphorylation Source:InterPro
Biological Process regulation of autophagy Source:UniProtKB
Biological Process regulation of glucose transmembrane transport Source:BHF-UCLBy Similarity
Biological Process regulation of MAP kinase activity Source:UniProtKB1 Publication
Biological Process response to endoplasmic reticulum stress Source:UniProtKB1 Publication
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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