Mouse Anti-TRMT2A Recombinant Antibody (CBYJT-4869) (CBMAB-T4415-YJ)

Name: Mouse Anti-TRMT2A Recombinant Antibody (CBYJT-4869)
SKU: CBMAB-T4415-YJ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBYJT-4869

Application

WB, IHC, IF, FC

Immunogen

Full length human recombinant protein of human TRMT2A(NP_073564) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)

Introduction

TRMT2A is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers.

Entrez Gene ID

27037

UniProt ID

Q8IZ69

Alternative Names

TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9c Protein; HTF9C; TRNA Methyltransferase 2 Homolog A (S. Cerevisiae); TRNA (Uracil-5-)-Methyltransferase Homolog A; TRM2 TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9C; EC 2.1.1.-

Function

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs (PubMed:31361898, PubMed:33799331, PubMed:34556860).
Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs (PubMed:31361898, PubMed:33799331).
Also able to mediate the formation of 5-methyl-uridine in some mRNAs (PubMed:34123281).

Biological Process

Biological Process mRNA processing Source:UniProtKB-KW

Cellular Location

Cytoplasm, cytosol

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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