Mouse Anti-TTF1 Recombinant Antibody (IHC414) (CBMAB-MA255-YC)

Name: Mouse Anti-TTF1 Recombinant Antibody (IHC414)
SKU: CBMAB-MA255-YC
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

IHC414

Application

IHC-P, ELISA, IHC (1:100 - 1:200)

Immunogen

Recombinant Human TTF-1

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

Tris buffer pH 7.3 - 7.7, 1% BSA, < 0.1% Sodium Azide

Storage

Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

More Infomation

Target

Full Name

TTF1 Transcription Termination Factor 1

Introduction

TTF1 is a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions.

Entrez Gene ID

7270

UniProt ID

Q15361

Alternative Names

Transcription Termination Factor 1; Transcription Termination Factor, RNA Polymerase I; TTF-1; TTF-I; RNA Polymerase I Termination Factor; Transcription Termination Factor I;

Function

Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.

Biological Process

DNA-templated transcription, termination1 PublicationNAS:UniProtKB
Negative regulation of DNA replicationIEA:UniProtKB-KW
Termination of RNA polymerase I transcriptionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Nucleus, nucleolus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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