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Mouse Anti-TUBB1 Recombinant Antibody (CBYJT-5285) (CBMAB-T4875-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TUBB1 (Tubulin Beta 1 Class VI). The antibody can be used for immunoassay techniques, such as WB, IF.
See all TUBB1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-5285
Antibody Isotype
IgG2a
Application
WB, IF

Basic Information

Immunogen
Full length human recombinant protein of human TUBB1(NP_110400) produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.3, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TUBB1 Gene(Protein Coding) Tubulin Beta 1 Class VI
Introduction
TUBB1 is a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. TUBB1 is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in TUBB1 is associated with autosomal dominant macrothrombocytopenia.
Entrez Gene ID
81027
UniProt ID
Q9H4B7
Alternative Names
Tubulin Beta 1 Class VI; Class VI Beta-Tubulin; Tubulin, Beta 1
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Biological Process
Biological Process microtubule cytoskeleton organization Source:GO_Central1 Publication
Biological Process mitotic cell cycle Source:GO_Central1 Publication
Biological Process spindle assembly Source:Ensembl
Cellular Location
Cytoplasm, cytoskeleton
Involvement in disease
Cytoplasm, cytoskeleton:
A congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
PTM
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866).
Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Glutamylation is also involved in cilia motility (By similarity).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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