Mouse Anti-UBE2E2 Recombinant Antibody (CBFYU-098) (CBMAB-U0098-FY)

Name: Mouse Anti-UBE2E2 Recombinant Antibody (CBFYU-098)
SKU: CBMAB-U0098-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

CBFYU-098

Application

ELISA, IF, WB

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 kD. Immunogen sequence: MSTEAQRVDD SPSTSGGSSD GDQRESVQQE PEREQVQPKK KEGKISSKTA AKLSTSAKRI QKELAEITLD PPPNCSAGPK GDNIYEWRST ILGPPGSVYE GGVFFLDITF SPDYPFKPPK VTFRTRIYHC NINSQGVICL DILKDNWSPA LTISKVLLSI CSLLTDCNPA DPLVGSIATQ YMTNRAEHDR MARQWTKRYA T.

Specificity

Human

Antibody Isotype

IgG1, к

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-201

More Infomation

Target

Full Name

UBE2E2

Introduction

UBE2E2 (Ubiquitin Conjugating Enzyme E2 E2) accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-11- and Lys-48-, as well as Lys-63-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein. Among its related pathways are Class I MHC mediated antigen processing and presentation and Ubiquitin mediated proteolysis. GO annotations related to this gene include ligase activity and acid-amino acid ligase activity.

Entrez Gene ID

7325

UniProt ID

Q96LR5

Alternative Names

Ubiquitin-Conjugating Enzyme E2E 2; Yeast UBC4/5; Ubiquitin-Conjugating Enzyme E2E 2; UbcH8; Ubiquitin Carrier Protein E2; Ubiquitin-Protein Ligase E2; UBCH8; EC 6.3.2.19; FLJ25157;

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.

Biological Process

Biological Process cellular response to DNA damage stimulus Source:MGI1 Publication
Biological Process ISG15-protein conjugation Source:HGNC-UCL1 Publication
Biological Process positive regulation of G1/S transition of mitotic cell cycle Source:MGI1 Publication
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication

Cellular Location

Nucleus

PTM

Autoubiquitinated in vitro.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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