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Mouse Anti-UBE2T Recombinant Antibody (2A12-4F11) (CBMAB-A9730-LY)

The product is antibody recognizes UBE2T. The antibody 2A12-4F11 immunoassay techniques such as: WB, ELISA.
See all UBE2T antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2A12-4F11
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
UBE2T (AAH04152, 1 a.a. ~ 197 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ubiquitin Conjugating Enzyme E2 T
Introduction
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM
Entrez Gene ID
29089
UniProt ID
Q9NPD8
Alternative Names
HSPC150; PIG50
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106).
Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784).
May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602).
In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).
Biological Process
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process DNA repair Source:UniProtKB1 Publication
Biological Process protein autoubiquitination Source:UniProtKB2 Publications
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K27-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K29-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB3 Publications
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Cellular Location
Nucleus
Accumulates to chromatin.
Involvement in disease
Fanconi anemia complementation group T (FANCT):
A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
PTM
Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645).
In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657).
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For research use only. Not intended for any clinical use.

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