Rabbit Anti-UBE2T Recombinant Antibody (CBFYH-3673) (CBMAB-H4235-FY)

Name: Rabbit Anti-UBE2T Recombinant Antibody (CBFYH-3673)
SKU: CBMAB-H4235-FY
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Rabbit

Clone

CBFYH-3673

Application

WB, IP, IF

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Ubiquitin Conjugating Enzyme E2 T

Introduction

The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

29089

UniProt ID

Q9NPD8

Alternative Names

Ubiquitin Conjugating Enzyme E2 T; Cell Proliferation-Inducing Gene 50 Protein; E2 Ubiquitin-Conjugating Enzyme T; Ubiquitin Carrier Protein T; Ubiquitin-Protein Ligase T; HSPC150 Protein Similar To Ubiquitin-Conjugating Enzyme; Ubiquitin-Conjugating Enzyme E2T (Putative); Ubiquitin-Conjugating Enzyme E2 T

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106).
Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784).
May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602).
In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).

Biological Process

Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process DNA repair Source:UniProtKB1 Publication
Biological Process protein autoubiquitination Source:UniProtKB2 Publications
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K27-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K29-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB3 Publications
Biological Process protein polyubiquitination Source:GO_Central1 Publication

Cellular Location

Nucleus
Accumulates to chromatin.

Involvement in disease

Fanconi anemia complementation group T (FANCT):
A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

PTM

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645).
In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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