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Mouse Anti-UBE2T Recombinant Antibody (CBFYU-158) (CBMAB-U0158-FY)

This product is mouse antibody that recognizes UBE2T. The antibody CBFYU-158 can be used for immunoassay techniques such as: WB, IF.
See all UBE2T antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYU-158
Antibody Isotype
IgG2a
Application
WB, IF

Basic Information

Immunogen
Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ubiquitin Conjugating Enzyme E2 T
Introduction
The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
29089
UniProt ID
Q9NPD8
Alternative Names
FANCT; PIG50; HSPC150
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106).
Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784).
May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602).
In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).
Biological Process
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process DNA repair Source:UniProtKB1 Publication
Biological Process protein autoubiquitination Source:UniProtKB2 Publications
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K27-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K29-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB3 Publications
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Cellular Location
Nucleus
Accumulates to chromatin.
Involvement in disease
Fanconi anemia complementation group T (FANCT):
A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
PTM
Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645).
In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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