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Mouse Anti-USP12 (aa 61-251) Recombinant Antibody (1E3) (CBMAB-U0029-CQ)

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Basic Information

Host Animal
Mouse
Clone
1E3
Application
WB
Immunogen
Recombinant protein fragment between amino acids 61-251 of human USP12 produced in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Purity
>95% as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 61-251
More Infomation

Target

Full Name
Ubiquitin Specific Peptidase 12
Introduction
Activated by interaction with WDR20 and WDR48 through different allosteric mechanisms.
Entrez Gene ID
219333
UniProt ID
O75317
Alternative Names
UBH1; USP12L1
Function
Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR20 and WDR48 to have a high activity (PubMed:19075014, PubMed:27373336).
Not involved in deubiquitination of monoubiquitinated FANCD2 (PubMed:19075014).
In complex with WDR48, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035).
Biological Process
Biological Process protein deubiquitination Source:UniProtKB2 Publications
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
cytosol
nucleoplasm
nucleus
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-USP12 Recombinant Antibody (2A6) (CAT#: CBMAB-U0044-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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