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Mouse Anti-USP16 (aa 137-466) Recombinant Antibody (1B4) (CBMAB-U0020-CQ)

This product is a mouse antibody that recognizes USP16 (aa 137-466). The antibody 1B4 can be used for immunoassay techniques such as: WB.
See all USP16 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B4
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Recombinant protein fragment between amino acids 137-466 of human USP16 produced in E. coli
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.8 mg/mL
Purity
>95% as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 137-466

Target

Full Name
USP16
Introduction
This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis.
Entrez Gene ID
10600
UniProt ID
Q9Y5T5
Alternative Names
Ubp-M
Function
Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
Biological Process
Biological Process cell division Source:UniProtKB-KW
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process chromatin organization Source:UniProtKB-KW
Biological Process histone deubiquitination Source:UniProtKB1 Publication
Biological Process histone H2A K63-linked deubiquitination Source:UniProtKB1 Publication
Biological Process mitotic cell cycle Source:UniProtKB1 Publication
Biological Process mitotic nuclear division Source:UniProtKB-UniRule
Biological Process monoubiquitinated histone H2A deubiquitination Source:UniProtKB
Biological Process positive regulation of DNA-templated transcription Source:UniProtKB1 Publication
Biological Process positive regulation of transcription by RNA polymerase II Source:UniProtKB
Biological Process positive regulation of translational elongation Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:Reactome
Biological Process protein homotetramerization Source:UniProtKB1 Publication
Biological Process regulation of cell cycle Source:InterPro
Biological Process regulation of transcription by RNA polymerase II Source:UniProtKB
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Nucleus
Involvement in disease
A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1.
PTM
Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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